Journal: Brain, behavior, and immunity
Article Title: Stress-induced depressive-like behavior in male rats is associated with microglial activation and inflammation dysregulation in the hippocampus in adulthood.
doi: 10.1016/j.bbi.2021.10.018
Figure Lengend Snippet: Fig. 4. Morphological analysis of hippocampal microglia. To evaluate microglial activation, images of IBA-1-positive microglia in three different subregions of the dorsal hippocampus (CA1, CA3 and DG) were acquired, and microglial morphology analyzed. (A) Representative images showing IBA-1-positive microglia in the CA1 subregion in green (left). IMARIS 3D arborization (center) and IMARIS convexHull (right) visualizations of H-CSR CTR and PPS rats (scale bar: 30 µm). (B) Radar chart containing the average results of the most relevant morphological features analyzed. (C) Quantitative analysis of hippocampal microglial morphology. Results represent the average values of the 3 subregions. Each dot represents an animal (N: N-CSR = 18 (9 CTR and 9 PPS); H-CSR = 24 (12 CTR and 12 PPS)). On average, more than 20 cells per rat were analyzed for soma volume, arborization volume, convexHull volume, max. branch length and total number of branches. Results are expressed as the mean ± SEM. Two-way ANOVA followed by Sidak’s multiple comparisons test, *p < 0.05. (D-E) Pearson correlation analyses were performed by comparing significantly altered morphological features (arborization volume and total number of branches) and IBA-1 expression (D) and arborization volume and % of time spent floating (FST) (E). Correlations were performed considering all animals (N = 42). The coefficient r and p-value for each correlation are presented in a box. Statistically significant correlations are highlighted in green (p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Article Snippet: Finally, the masks of the Skeleton and 3D Convex Hull were sent back to the Imaris Scene, and a results file was created with volumes and calculated ratios.
Techniques: Activation Assay, Expressing